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Reaction buffers for use in experiments involving modifying enzymes. Different buffer solutions create optimal reaction conditions for specific modifying enzymes, or facilitate multiple enzymatic reactions.
Proteinase K is a highly reactive nonspecific serine protease that belongs to the subtilisin family of proteins It cleaves at the carboxylic acid side of aliphatic aromatic or hydrophobic amino acids Proteinase K is capable of inactivating RNases and DNases and is used in the isolation or preparation of high molecular weight nucleic acids Proteinase K is also useful for helping to characterize enzymes due to its cleavage specificity This enzyme was designated proteinase K because of its ability to hydrolyze keratin Proteinase K is stable in a wide variety of detergents and buffer salts and at various temperatures and pH The isoelectric point of proteinase K is 8 9
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New England Biolabs supplies a reaction buffer with all of its enzymes. At a 1X concentration this reaction buffer assures optimal activity of T3, T7, Hi-T4 and Salt-T4 DNA Ligases .
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Proteinase K (PK) from fungi Tritirachium album encodes a 40 kDa protein. The N-terminal propeptide region shows homology with bacterial subtilisin. PK crystallizes even under microgravity conditions on the space shuttle mission. PK is an effective protein system for studying protein engineering process.
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Waters TA Instruments Quench Cooling Accessory (QCA) enables cooling from -180°C to 550°C for Discovery DSC series. DSC 25P supports down to -130°C. For quench cooling of autosampler-enabled Discovery DSCs, the following parts are also required: P/N: 973061.903 Manual Insulated Cover and 973252.001 Manual Silver Lid.
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